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Llébres et al. 2017 - Poster scientifique - Biosynthèse de l'arginine et utilisation chez l'embryon de pin maritime

Arginine biosynthesis and utilization in maritime pine

FEBS3+, 1st Joint Meeting of the French-Portuguese-Spanish Biochemical and Molecular Biology Societies, XL SEBBM Congress, Oct. 23-26, 2017, Barcelona, Spain. Poster P13-12, p. 120

Llebrés MT, Pascual MB, Debille S, Trontin JF, Harvengt L, Avila C, Cánovas FM

Collaboration Univ. Málaga (Espagne), FCBA

Support technique XYLOBIOTECH (site de Pierroton) :

Culture in vitro


Vegetative propagation through somatic embryogenesis in combination
with the cryopreservation of embryogenic lines is a major tool in conifer
biotechnology. An important process during the maturation phase of
embryogenesis is the biosynthesis and deposition of storage proteins. The
accumulation of some abundant storage proteins in maturing cotyledonary
somatic embryos (SE) is much lower than in mature zygotic embryos (ZE)
showing an important infl uence of storage compounds on the quality of
SE. Arginine constitutes a large portion of the amino acid pool in storage
proteins of conifers and therefore arginine biosynthesis and utiization is a
relevant metabolic pathway during pine embryogenesis and early growth.
Research in our laboratory is focused on maritime pine (Pinus pinaster
Ait.), a broadly planted conifer species in France, Spain and Portugal where
it is distributed over approximately 4 million hectares. This conifer species
is also one of the most advanced model trees for genetic and phenotypic
studies and a large number of molecular and transcriptomic resources are
currently available.
With the aim to understand the molecular basis of the diff erential
accumulation of storage proteins in SE and ZE, the arginine metabolic
pathway has been studied in maritime pine, in collaboration with the French
private institute FCBA. A general overview of this research programme
will be presented and discussed. The knowledge acquired from our studies
will help to refi ne biotechnological procedures for clonal propagation of
conifers via somatic embryogenesis.